1. Januar 2021
SERVA - Tools for Western blotting
Western blotting (protein blotting or immunoblotting) is a rapid and sensitive assay for detection and characterization of proteins. It is based on the principle of immunochromatography where proteins are separated into polyacrylamide gel according to their molecular weight.
5-minute blocking – for best results in Western blotting experiments BlueBlock PF
- Complete blocking of the membrane surface in only 5 minutes
- For background free antibody detection reactions
- Protein-free, 10fold concentrated solution
- Suitable for all detection methods and ELISA
Blue Block PF
From protein to membrane in only 40 minutes
Combine BlueBlock PF with SERVAGel™ HSE mini vertical ready-to-use gels for the separation of your samples in 20 minutes (10cm x 10cm with 10, 12 or 15 sample wells) and the Xpress Botting Kit for homogeneous and complete transfer in 15 minutes onto NC or PVDF membranes:
- SERVAGel™ HSE - 20 min
- Xpress Blotting - 15 min
- BlueBlock PF - 5 min
- Total process done - 40 min
SERVAGel™ Neutral HSE
* Prices are subject to variable conditions, ex works and excl. VAT
Xpress Blotting Kit
for fast Semi-Dry Western Blotting of 10 vertical mini SDS PAGE gels.
Kit contains:
250ml of 10x SERVA Xpress Blotting Buffer, 20x Blotting Fleece sheets (size 80x85 mm) and 10x Connection Paper (size 80x85 mm)
BlueClear SB for antibody stripping
- Ready-to-use buffer for efficient stripping of high-affinity antibodies
- Gentle, fast, simple – incubate the membrane for 30 minutes at RT
- Without β-mercaptoethanol or DTT
- Suitable for nitrocellulose and PVDF membranes
SERVA VisiBlot Standard I
- Ready-to-use, no reconstitution, further dilution or heating required
- Prestained bands for monitoring electrophoresis and membrane transfer
- Visualization of marker proteins on Western Blots by horseradish peroxidase or alkaline phosphatase-based immune-detection methods (7 bands at 25 kDa, 40 kDa, 60 kDa, 75 kDa, 90 kDa, 120 kDa, and 150 kDa)
- Molecular weight determination of proteins detected on transfer membrane (3 bands at 25 kDa, 45 kDa, and 85 kDa)
- For 100 lanes (mini gel format)
You’ll find more information in Western Blotting brochure from SERVA.